Content of cysteine, reduced and oxidized glutathione in spermatozoa of representatives of Acipenseriformes (Acipenser baerii and A. ruthenus) as well as teleosts (Perca fluviatilis and Sander lucioperca)
Identifieur interne : 000B17 ( Main/Exploration ); précédent : 000B16; suivant : 000B18Content of cysteine, reduced and oxidized glutathione in spermatozoa of representatives of Acipenseriformes (Acipenser baerii and A. ruthenus) as well as teleosts (Perca fluviatilis and Sander lucioperca)
Auteurs : K. Stejskal [République tchèque] ; Z. Svobodova [République tchèque] ; I. Fabrik [République tchèque] ; V. Adam [République tchèque] ; M. Beklova [République tchèque] ; M. Rodina [République tchèque] ; R. Kizek [République tchèque]Source :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2008-08.
Abstract
Glutathione belongs to a vital intra‐ and extra‐cellular protective antioxidant and is found almost exclusively in its reduced form. The ratio between its reduced and oxidized within cells is often used as a marker of cellular toxicity. The objectives of the study were to (i) determine both the reduced (GSH) and oxidized glutathione (GSSG) and cysteine (Cys) in the sperm of the Acipenser baerii and Acipenser ruthenus, as well as in Perca fluviatilis and Sander lucioperca, and (ii) to demonstrate the differences in concentration levels between representatives of acipenseriform and teleost species. High performance liquid chromatography with electrochemical detection was employed. The average content of the thiols determined in the sperm samples were as follows: Acipenser baerii (cysteine 55 ± 8 μg ml−1; GSH 126 ± 19 μg ml−1; GSSG 49 ± 7 μg ml−1), Acipenser ruthenus (cysteine 62 ± 9 μg ml−1; GSH 768 ± 115 μg ml−1; GSSG 180 ± 16 μg ml−1), Sander lucioperca (cysteine 251 ± 38 μg ml−1; GSH 185 ± 28 μg ml−1; GSSG 93 ± 14 μg ml−1), Perca fluviatilis (cysteine 281 ± 42 μg ml−1; GSH 496 ± 74 μg ml−1; GSSG 138 ± 21 μg ml−1). Based on the results obtained it can be concluded that this method is sensitive and selective for the determination of these compounds in real samples. Results revealed differences in cysteine content between species of the two systematic categories but also showed that ratios between GSH and GSSG can vary between species while potentially predict oxidative stress in fish sperm.
Url:
DOI: 10.1111/j.1439-0426.2008.01128.x
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000395
- to stream Istex, to step Curation: 000394
- to stream Istex, to step Checkpoint: 000539
- to stream Main, to step Merge: 000B39
- to stream Main, to step Curation: 000B17
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Content of cysteine, reduced and oxidized glutathione in spermatozoa of representatives of Acipenseriformes (Acipenser baerii and A. ruthenus) as well as teleosts (Perca fluviatilis and Sander lucioperca)</title><author><name sortKey="Stejskal, K" sort="Stejskal, K" uniqKey="Stejskal K" first="K." last="Stejskal">K. Stejskal</name></author><author><name sortKey="Svobodova, Z" sort="Svobodova, Z" uniqKey="Svobodova Z" first="Z." last="Svobodova">Z. Svobodova</name></author><author><name sortKey="Fabrik, I" sort="Fabrik, I" uniqKey="Fabrik I" first="I." last="Fabrik">I. Fabrik</name></author><author><name sortKey="Adam, V" sort="Adam, V" uniqKey="Adam V" first="V." last="Adam">V. Adam</name></author><author><name sortKey="Beklova, M" sort="Beklova, M" uniqKey="Beklova M" first="M." last="Beklova">M. Beklova</name></author><author><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M." last="Rodina">M. Rodina</name></author><author><name sortKey="Kizek, R" sort="Kizek, R" uniqKey="Kizek R" first="R." last="Kizek">R. Kizek</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:266F3156C4B8DA58290E46B0DD613655449D4AB7</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1111/j.1439-0426.2008.01128.x</idno><idno type="url">https://api.istex.fr/document/266F3156C4B8DA58290E46B0DD613655449D4AB7/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000395</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000395</idno><idno type="wicri:Area/Istex/Curation">000394</idno><idno type="wicri:Area/Istex/Checkpoint">000539</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000539</idno><idno type="wicri:doubleKey">0175-8659:2008:Stejskal K:content:of:cysteine</idno><idno type="wicri:Area/Main/Merge">000B39</idno><idno type="wicri:Area/Main/Curation">000B17</idno><idno type="wicri:Area/Main/Exploration">000B17</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Content of cysteine, reduced and oxidized glutathione in spermatozoa of representatives of Acipenseriformes (Acipenser baerii and A. ruthenus) as well as teleosts (Perca fluviatilis and Sander lucioperca)</title><author><name sortKey="Stejskal, K" sort="Stejskal, K" uniqKey="Stejskal K" first="K." last="Stejskal">K. Stejskal</name><affiliation wicri:level="3"><country>République tchèque</country><placeName><settlement type="city">Brno</settlement><region>Moravie</region></placeName><wicri:orgArea>Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry</wicri:orgArea></affiliation><affiliation wicri:level="3"><country>République tchèque</country><placeName><settlement type="city">Brno</settlement><region>Moravie</region></placeName><wicri:orgArea>Department of Biochemistry, Faculty of Science, Masaryk University</wicri:orgArea></affiliation></author><author><name sortKey="Svobodova, Z" sort="Svobodova, Z" uniqKey="Svobodova Z" first="Z." last="Svobodova">Z. Svobodova</name><affiliation></affiliation><affiliation wicri:level="3"><country>République tchèque</country><placeName><settlement type="city">Brno</settlement><region>Moravie</region></placeName><wicri:orgArea>Institute of Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences</wicri:orgArea></affiliation></author><author><name sortKey="Fabrik, I" sort="Fabrik, I" uniqKey="Fabrik I" first="I." last="Fabrik">I. Fabrik</name><affiliation wicri:level="3"><country>République tchèque</country><placeName><settlement type="city">Brno</settlement><region>Moravie</region></placeName><wicri:orgArea>Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry</wicri:orgArea></affiliation><affiliation wicri:level="3"><country>République tchèque</country><placeName><settlement type="city">Brno</settlement><region>Moravie</region></placeName><wicri:orgArea>Department of Biochemistry, Faculty of Science, Masaryk University</wicri:orgArea></affiliation></author><author><name sortKey="Adam, V" sort="Adam, V" uniqKey="Adam V" first="V." last="Adam">V. Adam</name><affiliation wicri:level="3"><country>République tchèque</country><placeName><settlement type="city">Brno</settlement><region>Moravie</region></placeName><wicri:orgArea>Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry</wicri:orgArea></affiliation><affiliation wicri:level="3"><country>République tchèque</country><placeName><settlement type="city">Brno</settlement><region>Moravie</region></placeName><wicri:orgArea>Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry</wicri:orgArea></affiliation></author><author><name sortKey="Beklova, M" sort="Beklova, M" uniqKey="Beklova M" first="M." last="Beklova">M. Beklova</name><affiliation wicri:level="3"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno</wicri:regionArea><placeName><settlement type="city">Brno</settlement><region>Moravie</region></placeName></affiliation></author><author><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M." last="Rodina">M. Rodina</name><affiliation wicri:level="3"><country>République tchèque</country><placeName><settlement type="city">Brno</settlement><region>Moravie</region></placeName><wicri:orgArea>Institute of Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences</wicri:orgArea></affiliation></author><author><name sortKey="Kizek, R" sort="Kizek, R" uniqKey="Kizek R" first="R." last="Kizek">R. Kizek</name><affiliation wicri:level="3"><country>République tchèque</country><placeName><settlement type="city">Brno</settlement><region>Moravie</region></placeName><wicri:orgArea>Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry</wicri:orgArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Applied Ichthyology</title><idno type="ISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2008-08">2008-08</date><biblScope unit="volume">24</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="519">519</biblScope><biblScope unit="page" to="521">521</biblScope></imprint><idno type="ISSN">0175-8659</idno></series><idno type="istex">266F3156C4B8DA58290E46B0DD613655449D4AB7</idno><idno type="DOI">10.1111/j.1439-0426.2008.01128.x</idno><idno type="ArticleID">JAI1128</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-8659</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Glutathione belongs to a vital intra‐ and extra‐cellular protective antioxidant and is found almost exclusively in its reduced form. The ratio between its reduced and oxidized within cells is often used as a marker of cellular toxicity. The objectives of the study were to (i) determine both the reduced (GSH) and oxidized glutathione (GSSG) and cysteine (Cys) in the sperm of the Acipenser baerii and Acipenser ruthenus, as well as in Perca fluviatilis and Sander lucioperca, and (ii) to demonstrate the differences in concentration levels between representatives of acipenseriform and teleost species. High performance liquid chromatography with electrochemical detection was employed. The average content of the thiols determined in the sperm samples were as follows: Acipenser baerii (cysteine 55 ± 8 μg ml−1; GSH 126 ± 19 μg ml−1; GSSG 49 ± 7 μg ml−1), Acipenser ruthenus (cysteine 62 ± 9 μg ml−1; GSH 768 ± 115 μg ml−1; GSSG 180 ± 16 μg ml−1), Sander lucioperca (cysteine 251 ± 38 μg ml−1; GSH 185 ± 28 μg ml−1; GSSG 93 ± 14 μg ml−1), Perca fluviatilis (cysteine 281 ± 42 μg ml−1; GSH 496 ± 74 μg ml−1; GSSG 138 ± 21 μg ml−1). Based on the results obtained it can be concluded that this method is sensitive and selective for the determination of these compounds in real samples. Results revealed differences in cysteine content between species of the two systematic categories but also showed that ratios between GSH and GSSG can vary between species while potentially predict oxidative stress in fish sperm.</div></front></TEI><affiliations><list><country><li>République tchèque</li></country><region><li>Moravie</li></region><settlement><li>Brno</li></settlement></list><tree><country name="République tchèque"><region name="Moravie"><name sortKey="Stejskal, K" sort="Stejskal, K" uniqKey="Stejskal K" first="K." last="Stejskal">K. Stejskal</name></region><name sortKey="Adam, V" sort="Adam, V" uniqKey="Adam V" first="V." last="Adam">V. Adam</name><name sortKey="Adam, V" sort="Adam, V" uniqKey="Adam V" first="V." last="Adam">V. Adam</name><name sortKey="Beklova, M" sort="Beklova, M" uniqKey="Beklova M" first="M." last="Beklova">M. Beklova</name><name sortKey="Fabrik, I" sort="Fabrik, I" uniqKey="Fabrik I" first="I." last="Fabrik">I. Fabrik</name><name sortKey="Fabrik, I" sort="Fabrik, I" uniqKey="Fabrik I" first="I." last="Fabrik">I. Fabrik</name><name sortKey="Kizek, R" sort="Kizek, R" uniqKey="Kizek R" first="R." last="Kizek">R. Kizek</name><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M." last="Rodina">M. Rodina</name><name sortKey="Stejskal, K" sort="Stejskal, K" uniqKey="Stejskal K" first="K." last="Stejskal">K. Stejskal</name><name sortKey="Svobodova, Z" sort="Svobodova, Z" uniqKey="Svobodova Z" first="Z." last="Svobodova">Z. Svobodova</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B17 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000B17 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:266F3156C4B8DA58290E46B0DD613655449D4AB7
|texte= Content of cysteine, reduced and oxidized glutathione in spermatozoa of representatives of Acipenseriformes (Acipenser baerii and A. ruthenus) as well as teleosts (Perca fluviatilis and Sander lucioperca)
}}
| This area was generated with Dilib version V0.6.27. |  |